Treatments 1 and 2 had 20.5 and 46.6 times increase respectively in the number of Tubifex worms at the end of a 45-days period of culture. The experimental units were small aquariums (length = 30 cm width = 25 cm height = 20 cm) adjusted to 100 ml/minute flow rate of water after preparing substrate treatments. (The proportion of COW DUNG or lettuce to sand was 4:1 in all the treatments and the diameter of sand granules was 0.25 to 0.40 mm). The substrate treatments were organic fertilizer ( COW DUNG) + sand with a height of 3 cm (treatment 1) and minced lettuce + sand with the same height (treatment 2). We investigated the influence of culture substrate on biomass production of worms in a completely randomized design with two treatments (substrates) each with four replicates. Due to the risk of transferring pathogens, collection of these worms from natural environment is not recommended. ![]() ![]() Abstract Having a diversity of necessary amino acids and fatty acids (W6 & W3) Tubifex worm (Tubifex tubifex) is a valuable food source for many ornamental fish species as well as larva of other fishes.
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